Comparative practicability and analytical performances of Credo VitaPCR™ Flu A&B and Cepheid Xpert® Xpress Flu/RSV platforms

Diagn Microbiol Infect Dis. 2021 Aug;100(4):115381. doi: 10.1016/j.diagmicrobio.2021.115381. Epub 2021 Mar 22.

Abstract

To compare the practicability (usability and satisfaction) and analytical performances of VitaPCR™ Flu A&B Assay (Credo Diagnostics Biomedical Pte. Ltd., Singapore, Republic of Singapore) and Xpert® Xpress Flu/RSV kit (Cepheid, Sunnyvale, USA), two rapid point-of-care (POC) nucleic acid amplification tests (NAATs) by reference to multiplex RT-PCR for respiratory viruses. Nasopharyngeal swabs (n=117) were collected from patients with influenza-like illness in Paris, France. Thawed specimens were further analyzed with both NAATs. The usability was comparable for both NAATs. Satisfaction questionnaire was better for the VitaPCR™ platform for the short time of test result in 20 minutes. Both NAATs showed comparable sensitivities (VitaPCRTM: 95.0%; Xpert® Xpress: 97.5%) and specificities (100%) for influenza A/B RNA detection, with excellent reliability and accuracy between both NAATs. Both VitaPCR™ and Xpert® Xpress NAATs can be implemented in hospital setting as POC NAATs to rapidly detect influenza A/B RNA in symptomatic patients.

Keywords: Bedside testing; Cepheid Xpert® Xpress; Influenza A; Influenza B; Innovative approaches; NAAT; PCR; Point-of-care; Practicability; Sensitivity; Specificity; VitaPCR™.

Publication types

  • Comparative Study

MeSH terms

  • Humans
  • Influenza A virus / genetics
  • Influenza, Human / diagnosis
  • Influenza, Human / virology
  • Molecular Diagnostic Techniques / instrumentation*
  • Molecular Diagnostic Techniques / methods
  • Nasopharynx / virology
  • Point-of-Care Testing / standards
  • Reagent Kits, Diagnostic / standards*
  • Real-Time Polymerase Chain Reaction / instrumentation*
  • Real-Time Polymerase Chain Reaction / standards
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Viruses / classification
  • Viruses / genetics*
  • Viruses / isolation & purification

Substances

  • Reagent Kits, Diagnostic