Blood glucose, lactate, pyruvate, glycerol, 3-hydroxybutyrate and acetoacetate measurements in man using a centrifugal analyser with a fluorimetric attachment

J Clin Chem Clin Biochem. 1988 Mar;26(3):141-6. doi: 10.1515/cclm.1988.26.3.141.


Methods are described for the analysis of glucose, lactate, pyruvate, alanine, glycerol, 3-hydroxybutyrate and acetoacetate in perchloric acid extracts of human blood, using the Cobas Bio centrifugal analyser fitted with a fluorimetric attachment. Intra-assay and inter-assay coefficients of variation ranged from 1.9 to 7.9% and from 1.0 to 7.2% respectively. Correlation coefficients ranged from 0.96 to 0.99 against established continuous-flow and manual spectrophotometric methods. All seven metabolites can be measured using a single perchloric acid extract of 20 microliter of blood. The versatility of the assays is such that as little as 100 pmol pyruvate, 3-hydroxybutyrate or as much as 15 nmol glucose can be measured in the same 20 microliter extract.

MeSH terms

  • Acetoacetates / blood
  • Blood Glucose / analysis*
  • Centrifugation
  • Fluorometry
  • Glycerol / blood*
  • Humans
  • Hydroxybutyrates / blood*
  • Lactates / blood*
  • Pyruvates / blood*


  • Acetoacetates
  • Blood Glucose
  • Hydroxybutyrates
  • Lactates
  • Pyruvates
  • Glycerol