Increased Isolation of Extended-Spectrum Beta-Lactamase-Producing Escherichia coli From Community-Onset Urinary Tract Infection Cases in Uttarakhand, India

Nitin Kumar; Kuhu Chatterjee; Sangeeta Deka; Ravi Shankar; Deepjyoti Kalita

doi:10.7759/cureus.13837

Increased Isolation of Extended-Spectrum Beta-Lactamase-Producing Escherichia coli From Community-Onset Urinary Tract Infection Cases in Uttarakhand, India

Cureus. 2021 Mar 11;13(3):e13837. doi: 10.7759/cureus.13837.

Authors

Nitin Kumar¹, Kuhu Chatterjee², Sangeeta Deka³, Ravi Shankar², Deepjyoti Kalita²

Affiliations

¹ General Practice, All India Institute of Medical Sciences Rishikesh, Rishikesh, IND.
² Microbiology, All India Institute of Medical Sciences Rishikesh, Rishikesh, IND.
³ Microbiology, Fakhruddin Ali Ahmed Medical College and Hospital, Barpeta, IND.

Abstract

Background: Management of community-acquired urinary tract infection (CA-UTI) relies heavily on empirical antibiotic therapy. Knowledge of the proportion of drug-resistant isolates especially extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli (E. coli), and various risk factors for acquisition are essential.

Method: Outpatient-treated CA-UTI cases were enrolled (continuously for three months), and microbiological analysis of urine sample was performed for significant bacterial growth followed by identification of conventional and matrix-assisted laser desorption/ionization-time of flight (MALDI-ToF) spectrometry method. Subsequent drug resistance and phenotypic ESBL detection were as per guidelines of the Clinical Laboratory Standard Institute (CLSI, USA). Univariate and multivariate analyses (logistic regression) of known and relevant risk factors of ESBL E. coli were performed as per standard statistical technique, using the SPSS computer package (IBM Corp., Armonk, NY). Results: Two hundred and forty-one samples (of 694 samples) yielded significant growth. Sixty-one of 131 (46.6%) E. coli isolates were found to be ESBL producers. Non-beta-lactam antibiotic resistance in ESBL producers was high compared to non-ESBL producers (e.g., 88.5% vs 42.3% for quinolone resistance, 80.3% vs 34.3% for gentamicin resistance, etc.). Multivariate analysis (after univariate analysis detected probable factors of a likely ESBL model) indicated significant associations of ESBL-producing E. coli with advancing age (>55 years), prior hospitalization in last one year, use of antibiotics in previous six months, and presence of comorbid illness such as diabetes mellitus and chronic lung disease.

Conclusion: High proportion of our community-acquired uropathogens are ESBL-producing E. coli and likely resistant to important antimicrobial agents such as quinolones, gentamicin, etc. Factors like advancing age, prior hospitalization, and antibiotic use, as well as comorbidities such as diabetes and chronic lung disease, may be strongly associated with ESBL E. coli and should be rememberedwhile administering or preparing guidelines for empiric management of CA-UTI subjects.

Keywords: anti-microbial resistance; ca-uti; community-acquired urinary tract infection; drug resistance; esbl; extended spectrum beta-lactamase; urinary tract infection; uropathogen; uti.