A protocol for transposon insertion sequencing in Schizosaccharomyces pombe to identify factors that maintain heterochromatin

Feng Li; Stevephen Hung; Caroline Esnault; Henry L Levin

doi:10.1016/j.xpro.2021.100392

A protocol for transposon insertion sequencing in Schizosaccharomyces pombe to identify factors that maintain heterochromatin

STAR Protoc. 2021 Mar 23;2(2):100392. doi: 10.1016/j.xpro.2021.100392. eCollection 2021 Jun 18.

Authors

Feng Li¹, Stevephen Hung¹, Caroline Esnault², Henry L Levin¹

Affiliations

¹ Division of Molecular and Cellular Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA.
² Bioinformatics and Scientific Programming Core, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA.

Abstract

Transposon insertion sequencing (TIS) is a highly effective method used with bacteria to identify genes important for growth in any condition of interest. Previously, we adapted this method to identify essential genes of the yeast Schizosaccharomyces pombe. Here, we describe modifications used to identify genes necessary for the formation of centromeric heterochromatin. For complete details on the use and execution of this protocol, please refer to Lee et al. (2020).

Keywords: Genomics; High-throughput screening; Model organisms; Molecular biology; Sequencing.

Publication types

Research Support, N.I.H., Intramural

MeSH terms

Centromere / genetics
DNA Transposable Elements / genetics*
DNA, Fungal / genetics
Genes, Fungal / genetics*
Genomics
Heterochromatin / genetics*
High-Throughput Nucleotide Sequencing
Schizosaccharomyces / genetics*
Sequence Analysis, DNA / methods*

Substances

DNA Transposable Elements
DNA, Fungal
Heterochromatin