Protocol for measurement of calcium dysregulation in human induced pluripotent stem cell-derived dopaminergic neurons

STAR Protoc. 2021 Mar 23;2(2):100405. doi: 10.1016/j.xpro.2021.100405. eCollection 2021 Jun 18.


Calcium regulation is a critical process in neurons, and Ca2+ signaling is a major contributor to neurological disorders including Parkinson's disease (PD). Here, combining calcium imaging with whole-cell Ca2+ current recording, we provide a detailed protocol for measuring Ca2+ homeostasis in dopaminergic (DA) neurons derived from human induced pluripotent stem cells (hiPSCs). This approach can be applied to investigate the role of Ca2+ homeostasis in neuronal functionality as well as in disease processes. For complete details on the use and execution of this protocol, please refer to Kim et al. (2020).

Keywords: Cell Differentiation; Microscopy; Neuroscience; Stem Cells.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Calcium / metabolism*
  • Calcium Signaling / physiology*
  • Dopaminergic Neurons* / cytology
  • Dopaminergic Neurons* / pathology
  • Electrophysiology / methods*
  • Humans
  • Induced Pluripotent Stem Cells / cytology*
  • Molecular Imaging
  • Patch-Clamp Techniques / methods


  • Calcium