Protocol for isolation of adult mouse ear pinnae-derived primary fibroblasts

Juan I Bravo; Minhoo Kim; Bérénice A Benayoun

doi:10.1016/j.xpro.2021.100406

Protocol for isolation of adult mouse ear pinnae-derived primary fibroblasts

STAR Protoc. 2021 Mar 23;2(2):100406. doi: 10.1016/j.xpro.2021.100406. eCollection 2021 Jun 18.

Authors

Juan I Bravo^{1

2}, Minhoo Kim¹, Bérénice A Benayoun^{1

3

4

5

6}

Affiliations

¹ Leonard Davis School of Gerontology, University of Southern California, Los Angeles, CA 90089, USA.
² Graduate Program in the Biology of Aging, University of Southern California, Los Angeles, CA 90089, USA.
³ Neuroscience Graduate Program, University of Southern California, Los Angeles, CA 90089, USA.
⁴ USC Norris Comprehensive Cancer Center, Epigenetics and Gene Regulation, Los Angeles, CA 90089, USA.
⁵ Molecular and Computational Biology Department, USC Dornsife College of Letters, Arts and Sciences, Los Angeles, CA 90089, USA.
⁶ USC Stem Cell Initiative, Los Angeles, CA 90089, USA.

Abstract

Researchers need in vitro models that mirror the biology of organisms. Primary fibroblasts play essential roles in wound healing and are present in many tissues. They are widely used in studies of cell cycle control, reprogramming, and aging. Though extraction protocols exist, alternatives that maximize use of available resources are useful. Here, we present our protocol for extracting primary fibroblasts from adult mouse ear pinnae, an often-discarded source of primary cells, which consistently yield large, pure numbers of primary fibroblasts.

Keywords: Cell culture; Cell isolation.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Animals
Cell Culture Techniques / methods*
Cell Separation / methods*
Cells, Cultured
Ear Auricle / cytology*
Female
Fibroblasts / cytology*
Male
Mice
Mice, Inbred C57BL

Associated data

figshare/10.6084/m9.figshare.13661051.v1

Abstract

Publication types

MeSH terms

Associated data

Grants and funding