Sub millimetre flexible fibre probe for background and fluorescence free Raman spectroscopy

J Biophotonics. 2021 Oct;14(10):e202000488. doi: 10.1002/jbio.202000488. Epub 2021 Jul 4.


Using the shifted-excitation Raman difference spectroscopy technique and an optical fibre featuring a negative curvature excitation core and a coaxial ring of high numerical aperture collection cores, we have developed a portable, background and fluorescence free, endoscopic Raman probe. The probe consists of a single fibre with a diameter of less than 0.25 mm packaged in a sub-millimetre tubing, making it compatible with standard bronchoscopes. The Raman excitation light in the fibre is guided in air and therefore interacts little with silica, enabling an almost background free transmission of the excitation light. In addition, we used the shifted-excitation Raman difference spectroscopy technique and a tunable 785 nm laser to separate the fluorescence and the Raman spectrum from highly fluorescent samples, demonstrating the suitability of the probe for biomedical applications. Using this probe we also acquired fluorescence free human lung tissue data.

Keywords: Raman fibre probe; Raman spectroscopy; SERDS; hollow-core fibre.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Fluorescent Dyes*
  • Humans
  • Silicon Dioxide
  • Spectrum Analysis, Raman*


  • Fluorescent Dyes
  • Silicon Dioxide