Cytotoxic Activity in Broth-Culture Filtrates of Campylobacter Pylori

J Med Microbiol. 1988 Jun;26(2):93-9. doi: 10.1099/00222615-26-2-93.


Broth-culture filtrates of Campylobacter pylori induced non-lethal cytopathic effects in vitro in 7 of 9 mammalian cell lines tested. Transmission electronmicroscopy revealed that the response consisted of intracellular vacuolisation. Intestine 407 cells were among the most responsive and were used for routine assay. About 55% of isolates of C. pylori tested, originating from four geographic regions worldwide, produced cytotoxic activity. The activity was neutralisable by specific antisera to broth-culture filtrates or to sonicated bacteria but not by antisera to other bacterial preparations. Cytotoxic activity was heat-labile (70 degrees C for 30 min), was protease-sensitive and ammonium-sulphate precipitable. It did not pass through an ultrafiltration membrane with a nominal mol.-wt limit of 100 X 10(3). It was concluded that C. pylori can produce a factor that alters cultured cells in vitro. The relevance of this factor to the pathogenesis of gastritis associated with C. pylori remains to be determined.

MeSH terms

  • Animals
  • Bacterial Toxins / biosynthesis*
  • Campylobacter / immunology
  • Campylobacter / metabolism
  • Campylobacter / pathogenicity*
  • Cell Line
  • Culture Media
  • Cytotoxins / biosynthesis*
  • HeLa Cells
  • Humans
  • Immune Sera / immunology
  • Male
  • Microscopy, Electron
  • Rabbits
  • Vacuoles / ultrastructure
  • Vero Cells


  • Bacterial Toxins
  • Culture Media
  • Cytotoxins
  • Immune Sera