A pilot study of an anti-endotoxin Ig-enriched bovine colostrum to prevent experimental sepsis

Innate Immun. 2021 Apr;27(3):266-274. doi: 10.1177/17534259211007538.


Despite the dramatic increase in antimicrobial resistance, there is a dearth of antibiotics in development and few pharmaceutical companies working in the field. Further, any new antibiotics are likely to have a short shelf life. Ab-based interventions offer alternatives that are not likely to be circumvented by the widely prevalent antibiotic resistance genes. Bovine colostrum (BC)-the first milk after parturition, rich in nutrients and immune components-promotes gut integrity and modulates the gut microbiome. We developed a hyperimmune BC (HBC) enriched in Abs to a highly conserved LOS core region of Gram-negative bacteria by immunizing pregnant cows with a vaccine comprised of detoxified LOS from Escherichia coli O111 Rc (J5) mutant non-covalently complexed to group B meningococcal outer membrane protein (J5dLOS/OMP). This vaccine generated robust levels of anti-J5 LOS Ab in the colostrum. When given orally to neutropenic rats challenged orally with Pseudomonas aeruginosa, administration of HBC improved survival compared to non-immune rats, while both BC preparations improved survival compared to PBS controls. Elevated circulating endotoxin levels correlated with mortality. HBC and to a lesser extent non-immune BC reduced bacterial burden from the liver, lung, and spleen. We conclude that HBC and to a lesser extent BC may be effective supplements that improve outcome from lethal gut-derived disseminated infection and may reduce transmission of Gram-negative bacilli from the gastrointestinal tract.

Keywords: Antibody; Pseudomonas aeruginosa; antimicrobial resistance; bovine colostrum; endotoxin.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Bacterial / metabolism
  • Bacterial Load
  • Bacterial Outer Membrane Proteins / immunology
  • Bacterial Outer Membrane Proteins / metabolism*
  • Bacterial Vaccines / immunology*
  • Cattle
  • Endotoxins / immunology*
  • Escherichia coli / metabolism*
  • Immunoglobulin G / metabolism*
  • Immunoglobulins / metabolism*
  • Lipopolysaccharides / immunology
  • Models, Animal
  • Pilot Projects
  • Sepsis / immunology*


  • Antibodies, Bacterial
  • Bacterial Outer Membrane Proteins
  • Bacterial Vaccines
  • Endotoxins
  • Immunoglobulin G
  • Immunoglobulins
  • Lipopolysaccharides