Metatranscriptomic Analysis Reveals SARS-CoV-2 Mutations in Wastewater of the Frankfurt Metropolitan Area in Southern Germany

Shelesh Agrawal; Laura Orschler; Susanne Lackner

doi:10.1128/MRA.00280-21

Metatranscriptomic Analysis Reveals SARS-CoV-2 Mutations in Wastewater of the Frankfurt Metropolitan Area in Southern Germany

Microbiol Resour Announc. 2021 Apr 15;10(15):e00280-21. doi: 10.1128/MRA.00280-21.

Authors

Shelesh Agrawal¹, Laura Orschler², Susanne Lackner²

Affiliations

¹ Technical University of Darmstadt, Institute IWAR, Chair of Wastewater Engineering, Darmstadt, Germany s.agrawal@iwar.tu-darmstadt.de.
² Technical University of Darmstadt, Institute IWAR, Chair of Wastewater Engineering, Darmstadt, Germany.

Abstract

We report a sequencing analysis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in wastewater samples collected in the Frankfurt metropolitan area of Germany. The majority of the detected mutations have been identified only in clinical genomes outside Frankfurt, indicating that the sequencing of SARS-CoV-2 RNA in wastewater can provide insights into emerging variants in a city.