Effect of 1,25-dihydroxyvitamin D3 on cytosolic calcium in dispersed parathyroid cells

Kidney Int. 1988 Apr;33(4):850-4. doi: 10.1038/ki.1988.76.


We examined the effect of 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) on cytosolic calcium ([Ca]i) of dispersed bovine parathyroid cells, using the fluorescent dye indo-1. The addition of 10(-8) M 1,25-(OH)2D3 caused an increase in [Ca]i by 23.4 +/- 2.7% over a 10 minute period. There was a significant increase in [Ca]i within two minutes of the addition of 1,25-(OH)2D3. 1,25-(OH)2D3 increased [Ca]i in a dose-dependent manner and this occurred with as little as 10(-10) M. Neither 10(-7) M 25-(OH)D3 nor 10(-7) M 24, 25-(OH)2D3 caused a significant increase in [Ca]i. Chelation of extracellular calcium with EGTA blocked the 1,25-(OH)2D3-induced increase in [Ca]i, suggesting that the increase was mainly from extracellular calcium. Neither 10(-5) M verapamil nor 10(-4) M diltiazem blocked the 1,25-(OH)2D3-induced increase in [Ca]i. The present data suggest that 1,25-(OH)2D3 might modify membrane permeability to calcium independent of voltage-dependent calcium channels sensitive to verapamil or diltiazem. The rapid effect of 1,25-(OH)2D3 raises the possibility that its mechanism is independent of genome activation, perhaps attributable to direct interaction with components of the parathyroid cell plasma membrane.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Calcitriol / pharmacology*
  • Calcium / metabolism*
  • Cattle
  • Cytosol / metabolism*
  • Diltiazem / pharmacology
  • In Vitro Techniques
  • Indoles / therapeutic use
  • Parathyroid Glands / drug effects
  • Parathyroid Glands / metabolism*
  • Verapamil / pharmacology


  • Indoles
  • Verapamil
  • Diltiazem
  • Calcitriol
  • Calcium