Urine proteome changes in a chronic unpredictable mild stress (CUMS) mouse model of major depressive disorder

Yuhang Huan; Jing Wei; Tong Su; Youhe Gao

doi:10.1016/j.jpba.2021.114064

Urine proteome changes in a chronic unpredictable mild stress (CUMS) mouse model of major depressive disorder

J Pharm Biomed Anal. 2021 May 30:199:114064. doi: 10.1016/j.jpba.2021.114064. Epub 2021 Apr 6.

Authors

Yuhang Huan¹, Jing Wei², Tong Su³, Youhe Gao⁴

Affiliations

¹ Department of Biochemistry and Molecular Biology, Beijing Normal University, Gene Engineering Drug and Biotechnology Beijing Key Laboratory, Beijing, 100875, China. Electronic address: huanyuhang@mail.bnu.edu.cn.
² Department of Biochemistry and Molecular Biology, Beijing Normal University, Gene Engineering Drug and Biotechnology Beijing Key Laboratory, Beijing, 100875, China. Electronic address: weijingbnu@bnu.edu.cn.
³ State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences Chinese Academy of Medical Sciences and Peking Union Medical College, Neuroscience Center, Chinese Academy of Medical Sciences, Beijing, 100005, China. Electronic address: 13678917505@126.com.
⁴ Department of Biochemistry and Molecular Biology, Beijing Normal University, Gene Engineering Drug and Biotechnology Beijing Key Laboratory, Beijing, 100875, China. Electronic address: gaoyouhe@bnu.edu.cn.

PMID: 33862505
DOI: 10.1016/j.jpba.2021.114064

Abstract

Major depressive disorder (MDD) is a prevalent complex psychiatric disorder, and there are no effective biomarkers for clinical diagnosis. Urine is not subjected to homeostatic control, allowing it to reflect the sensitive changes that occur in various diseases. In this study, we examined the urine proteome changes in a chronic unpredictable mild stress mouse model of MDD. Male C57BL/6 mice were subjected to chronic unpredictable mild stress for 5 weeks. The tail suspension test and sucrose consumption test were then applied to evaluate depression-like behaviors. The urine proteomes on day 0 and day 36 in the CUMS group were profiled by liquid chromatography coupled with tandem mass spectrometry (LCMS/MS). A total of 36 differential proteins were identified, 19 of which have been associated with the pathogenic mechanisms of MDD. There was an average of two differential proteins that were identified through 1,048,574 random combination statistical analyses, indicating that at least 95 % of the differential proteins were reliable. The differential proteins were mainly associated with blood coagulation, inflammatory responses and central nervous system development. Our preliminary results indicated that the urine proteome can reflect changes associated with MDD in the CUMS model, which provides potential clues for the diagnosis of MDD.

Keywords: Biomarker; CUMS model; Major depressive disorder; Urine proteome.

MeSH terms

Animals
Depression
Depressive Disorder, Major*
Disease Models, Animal
Hippocampus
Male
Mice
Mice, Inbred C57BL
Proteome
Stress, Psychological

Substances

Proteome