Isolation of stromal vascular fraction cell suspensions from mouse and human adipose tissues for downstream applications

STAR Protoc. 2021 Mar 30;2(2):100422. doi: 10.1016/j.xpro.2021.100422. eCollection 2021 Jun 18.

Abstract

This protocol outlines a reliable and versatile approach to isolate stromal vascular fraction cells from different adipose tissues across human and mouse species. A number of downstream applications can then be performed to gain an appreciation of the functional activity of unique adipose tissue-resident cell populations. For complete details on the use and execution of this protocol, please refer to Macdougall et al. (2018).

Keywords: Cell Biology; Cell isolation; Flow Cytometry/Mass Cytometry; Immunology; Metabolism.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipose Tissue / cytology*
  • Animals
  • Cell Culture Techniques / methods*
  • Cell Separation / methods*
  • Cells, Cultured
  • Female
  • Humans
  • Male
  • Mice
  • Stromal Vascular Fraction / physiology*