Rapid Detection of Transposon-Induced Genome Rearrangements

Methods Mol Biol. 2021;2250:131-139. doi: 10.1007/978-1-0716-1134-0_13.


This chapter details the techniques used to detect transposon-induced genome rearrangements. Here, we describe a rapid DNA isolation technique, PCR amplification, and a novel High Efficiency Agarose Gel Electrophoresis Method (HEA-GEM).

Keywords: High efficiency agarose gel electrophoresis; Large population size; PCR; Plate DNA extraction; Rapid DNA isolation.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • DNA Transposable Elements*
  • DNA, Plant / genetics*
  • Electrophoresis, Agar Gel
  • Mutagenesis, Insertional
  • Oryza / genetics*
  • Polymerase Chain Reaction


  • DNA Transposable Elements
  • DNA, Plant