L-arginine is the physiological precursor for the formation of nitric oxide in endothelium-dependent relaxation

Biochem Biophys Res Commun. 1988 Jun 30;153(3):1251-6. doi: 10.1016/s0006-291x(88)81362-7.


The formation of nitric oxide (NO) from L-arginine by vascular endothelial cells and its relationship to endothelium-dependent relaxation of vascular rings was studied. The release of NO, measured by bioassay or chemiluminescence, from porcine aortic endothelial cells stimulated with bradykinin was enhanced by infusions of L-, but not D-arginine. The release of 15NO, determined by high resolution mass spectrometry, from L-guanidino 15N (99%) arginine was also observed, indicating that NO is formed from the terminal guanidino nitrogen atom(s) of L-arginine. L-NG-monomethyl arginine (L-NMMA), but not D-NMMA, inhibited both the generation of NO by endothelial cells in culture and the endothelium-dependent relaxation of rabbit aortic rings. Both these effects were reversed by L-arginine. These data indicate that L-arginine is the physiological precursor for the formation of NO which mediates endothelium-dependent relaxation.

MeSH terms

  • Animals
  • Arginine / metabolism*
  • Biological Assay
  • Bradykinin / pharmacology
  • Endothelium, Vascular / drug effects
  • Endothelium, Vascular / metabolism*
  • Isomerism
  • Luminescent Measurements
  • Nitric Oxide / metabolism*
  • Swine
  • Vasodilation*


  • Nitric Oxide
  • Arginine
  • Bradykinin