Colorimetric isothermal nucleic acid detection of SARS-CoV-2 with dye combination

Shanshan Wu; Xiyang Liu; Shenglong Ye; Jianmin Liu; Wei Zheng; Xue Dong; Xiushan Yin

doi:10.1016/j.heliyon.2021.e06886

Colorimetric isothermal nucleic acid detection of SARS-CoV-2 with dye combination

Heliyon. 2021 Apr;7(4):e06886. doi: 10.1016/j.heliyon.2021.e06886. Epub 2021 Apr 21.

Authors

Shanshan Wu¹, Xiyang Liu², Shenglong Ye², Jianmin Liu³, Wei Zheng⁴, Xue Dong³, Xiushan Yin^{1

2

5

6

7

8

9}

Affiliations

¹ Applied Biology Laboratory, College of Pharmaceutical and Biological Engineering, Shenyang University of Chemical Technology, 110142, Shenyang, China.
² Biotech & Biomedicine Science (Shenyang) Co. Ltd, Shenyang, 110000, China.
³ Shenyang Center for Disease Control and Prevention, 110031, Shenyang, Liaoning, China.
⁴ Liaoning Cancer Hospital and Institute, No. 44 Xiaoheyan Rd, Dadong District, ShenYang, 110042 China.
⁵ SciLifeLab, Department of Microbiology, Tumor and Cell Biology. Karolinska Institutet, Solna 17165, Sweden.
⁶ Pluri Biotech Co.Ltd, Xuzhou, 221001, China.
⁷ Nanog Biotech Co.Ltd, Shanghai, 200000, China.
⁸ Biotech & Biomedicine Science (Jiangxi) Co. Ltd, Ganzhou, 341000, China.
⁹ Department of Respiratory and Critical Care Medicine, Central Hospital Affiliated to Shenyang Medical College, Shenyang 110024, People's Republic of China.

Abstract

RT-LAMP detection of SARS-CoV-2 has been demonstrated to be a valuable diagnostic method for the diagnosis of COVID-19^1,2, which can rapidly screen carriers of the virus to effectively control the spread of the SARS-CoV-2. Here, we present a combination of dyes for isothermal detection of SARS-CoV-2 as a commercial alternative, with expanded colorimetric spectrum. We compared them with commercial reagents and proved their suitability and sensitivity through clinical RNA samples. In addition, together with commercial single dye indicators, we believe the expanded color spectrum developed here as an indicator of rapid detection will promote the diagnosis of COVID-19.

Keywords: Colorimetric detection; Dye combination; RT-LAMP; SARS-CoV-2.