Systematic study of SYBR green chromophore reveals major improvement with one heteroatom difference

J Mater Chem B. 2021 Apr 28;9(16):3484-3488. doi: 10.1039/d1tb00312g.

Abstract

Five nucleic acid binding cyanine dyes were synthesized and their photophysical properties were evaluated. Changing a single heteroatom in the chromophore causes major differences both in brightness and photostability between the dyes. With such alteration, the brightness of the chromophore increased two-fold compared to the one found in SYBR Green I.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Benzothiazoles / chemical synthesis
  • Benzothiazoles / chemistry*
  • DNA / analysis*
  • Diamines / chemical synthesis
  • Diamines / chemistry*
  • Fluorescent Dyes / chemical synthesis
  • Fluorescent Dyes / chemistry*
  • Molecular Structure
  • Quinolines / chemical synthesis
  • Quinolines / chemistry*
  • RNA / analysis*

Substances

  • Benzothiazoles
  • Diamines
  • Fluorescent Dyes
  • Quinolines
  • SYBR Green I
  • RNA
  • DNA