Polydatin Inhibits Cell Viability, Migration, and Invasion Through Suppressing the c-Myc Expression in Human Cervical Cancer

Longchang Bai; Yingkang Ma; Xue Wang; Qiongni Feng; Zhining Zhang; Sijie Wang; Huijie Zhang; Xinyu Lu; Yonghui Xu; Erhu Zhao; Hongjuan Cui

doi:10.3389/fcell.2021.587218

Polydatin Inhibits Cell Viability, Migration, and Invasion Through Suppressing the c-Myc Expression in Human Cervical Cancer

Front Cell Dev Biol. 2021 Apr 12:9:587218. doi: 10.3389/fcell.2021.587218. eCollection 2021.

Authors

Longchang Bai^{1

2

3}, Yingkang Ma^{1

2

3}, Xue Wang⁴, Qiongni Feng^{1

2}, Zhining Zhang^{1

2

3}, Sijie Wang^{1

2

3}, Huijie Zhang^{1

2

3}, Xinyu Lu^{1

2

3}, Yonghui Xu¹, Erhu Zhao^{1

5

6}, Hongjuan Cui^{1

5

3

6}

Affiliations

¹ State Key Laboratory of Silkworm Genome Biology, College of Sericulture, Textile and Biomass Sciences, Southwest University, Chongqing, China.
² Westa College, Southwest University, Chongqing, China.
³ Chongqing Engineering and Technology Research Center for Silk Biomaterials and Regenerative Medicine, Chongqing, China.
⁴ Chongqing General Hospital, University of Chinese Academy of Sciences, Chongqing, China.
⁵ Cancer Center, Medical Research Institute, Southwest University, Chongqing, China.
⁶ Engineering Research Center for Cancer Biomedical and Translational Medicine, Southwest University, Chongqing, China.

Abstract

Polydatin, an active ingredient from the roots of Polygonum cuspidatum, is considered to have protective effects on the cardiovascular system and liver. In this study, we demonstrated that polydatin has antitumor activity against human cervical cancer. Polydatin efficiently inhibited cervical cancer cell proliferation by regulating cell cycle-related proteins including p21, p27, CDK2, CDK4, Cyclin D1, and Cyclin E1. Furthermore, polydatin suppressed cell invasion and migration by regulating epithelial-mesenchymal transition (EMT) markers, including E-cadherin, N-cadherin, Snail and Slug. The c-Myc, as a proto-oncogene, is considered to be closely associated with the proliferation and metastasis of tumor cells. After polydatin treatment, the protein expression of c-Myc showed a significant decrease. Based on these data, we overexpressed c-Myc in cervical cancer cells and observed that the overexpression of c-Myc rescued the inhibitory effect of polydatin on cell proliferation and metastasis. These results indicated that polydatin can inhibit cell proliferation and metastasis through suppressing the c-Myc expression in human cervical cancer.

Keywords: c-Myc; cell viability; cervical cancer; migration and invasion; polydatin.