Efficacy of Souroubea-Platanus Dietary Supplement Containing Triterpenes in Beagle Dogs Using a Thunderstorm Noise-Induced Model of Fear and Anxiety

Molecules. 2021 Apr 3;26(7):2049. doi: 10.3390/molecules26072049.


A novel botanical dietary supplement, formulated as a chewable tablet containing a defined mixture of Souroubea spp. vine and Platanus spp. Bark, was tested as a canine anxiolytic for thunderstorm noise-induced stress (noise aversion). The tablet contained five highly stable triterpenes and delivered 10 mg of the active ingredient betulinic acid (BA) for an intended 1 mg/kg dose in a 10 kg dog. BA in tablets was stable for 30 months in storage at 23 °C. Efficacy of the tablets in reducing anxiety in dogs was assessed in a blinded, placebo-controlled study by recording changes in blood cortisol levels and measures of behavioral activity in response to recorded intermittent thunder. Sixty beagles were assigned into groups receiving: placebo, 0.5×, 1×, 2×, and 4× dose, or the positive control (diazepam), for five days. Reduction in anxiety measures was partially dose-dependent and the 1× dose was effective in reducing inactivity time (p = 0.0111) or increased activity time (p = 0.0299) compared with placebo, indicating a decrease in anxiety response. Cortisol measures also showed a dose-dependent reduction in cortisol in dogs treated with the test tablet.

Keywords: Platanus; Souroubea; anxiety; canines; dietary supplement; noise aversion; triterpenes.

MeSH terms

  • Animals
  • Anxiety / blood
  • Anxiety / therapy*
  • Dietary Supplements*
  • Disease Models, Animal
  • Dogs
  • Dose-Response Relationship, Drug
  • Ericales / chemistry*
  • Fear / drug effects*
  • Hydrocortisone / blood
  • Least-Squares Analysis
  • Magnoliopsida / chemistry*
  • Tablets
  • Triterpenes / chemistry
  • Triterpenes / pharmacology*


  • Tablets
  • Triterpenes
  • Hydrocortisone