Clonal growth characteristics of adult human prostatic epithelial cells

In Vitro Cell Dev Biol. 1988 Jun;24(6):530-6. doi: 10.1007/BF02629087.


The ability of human epithelial cells derived from adult prostatic tissues to undergo clonal growth in culture was examined. In a previously described serum-free culture system, such cells exhibited a density-dependent growth requirement. It was found that raising the level of one of the constituents of the culture medium, bovine pituitary extract, to 100 micrograms/ml permitted excellent clonal growth when as few as 100 cells were inoculated/60-mm2 dish. Raising the levels of supplements other than pituitary extract (cholera toxin, epidermal growth factor, hydrocortisone, or insulin) did not produce this result. The average colony-forming efficiency of cells derived from primary or early passage cultures was approximately 25%. When single cell suspensions were prepared from tissue isolates and directly analyzed for clonal growth, colony-forming efficiencies were approximately 5%, perhaps indicating the proportion of stem cells with proliferative potential in the original isolates. The colony-forming efficiency of a cell population derived from cancer tissue was not significantly different from those of populations derived from normal tissues.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Division
  • Cells, Cultured
  • Clone Cells / cytology
  • Colony-Forming Units Assay
  • Culture Media
  • Epithelial Cells
  • Fibroblast Growth Factor 2
  • Humans
  • Male
  • Nerve Tissue Proteins / pharmacology
  • Pituitary Gland / physiology
  • Prostate / cytology*
  • Prostatic Neoplasms / pathology
  • Stem Cells / pathology
  • Tissue Extracts / pharmacology
  • Tumor Cells, Cultured


  • Culture Media
  • Nerve Tissue Proteins
  • Tissue Extracts
  • Fibroblast Growth Factor 2