Probing membrane protein-lipid interactions

Curr Opin Struct Biol. 2021 Aug:69:78-85. doi: 10.1016/ Epub 2021 Apr 27.


Structure determination of membrane proteins has highlighted the many roles played by lipids in influencing overall protein architecture. It is now widely accepted that lipids surrounding membrane proteins play crucial roles by modulating their conformational, structural, and functional properties. Capturing often transient lipid interactions and defining their chemical identity, however, remains challenging. Recent advances in mass spectrometry have resolved questions concerning lipid interactions by providing the molecular composition of intact complexes in association with lipids. Together with other biophysical tools, a picture is emerging of the dynamic nature of lipid-mediated interactions and their effects on conformation, interactions, and signaling.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Cell Membrane
  • Mass Spectrometry
  • Membrane Lipids*
  • Membrane Proteins*


  • Membrane Lipids
  • Membrane Proteins