Quantifying molecular aggregation by super resolution microscopy within an excitatory synapse from mouse hippocampal neurons

STAR Protoc. 2021 Apr 14;2(2):100470. doi: 10.1016/j.xpro.2021.100470. eCollection 2021 Jun 18.


Super-resolution microscopy (SRM) has been widely adopted to probe molecular distribution at excitatory synapses. We present an SRM paradigm to evaluate the nanoscale organization heterogeneity between neuronal subcompartments. Using mouse hippocampal neurons, we describe the identification of the morphological characteristics of nanodomains within functional zones of a single excitatory synapse. This information can be used to correlate structure and function at molecular resolution in single synapses. The protocol can be applied to immunocytochemical/histochemical samples across different imaging paradigms. For complete details on the use and execution of this protocol, please refer to Kedia et al. (2021).

Keywords: Cell culture; Microscopy; Neuroscience.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Hippocampus / cytology*
  • Hippocampus / metabolism*
  • Mice
  • Microscopy*
  • Neurons / cytology*
  • Neurons / metabolism*
  • Synapses / metabolism*