Cardamonin Attenuates Experimental Colitis and Associated Colorectal Cancer

Biomolecules. 2021 Apr 29;11(5):661. doi: 10.3390/biom11050661.


Cardamonin is a naturally occurring chalcone, majorly from the Zingiberaceae family, which includes a wide range of spices from India. Herein, we investigated the anti-inflammatory property of cardamonin using different in vitro and in vivo systems. In RAW 264.7 cells, treatment with cardamonin showed a reduced nitrous oxide production without affecting the cell viability and decreased the expression of iNOS, TNF-α, and IL-6, and inhibited NF-kB signaling which emphasizes the role of cardamonin as an anti-inflammatory molecule. In a mouse model of dextran sodium sulfate (DSS)-induced colitis, cardamonin treatment protected the mice from colitis. Subsequently, we evaluated the therapeutic potential of this chalcone in a colitis-associated colon cancer model. We performed microRNA profiling in the different groups and observed that cardamonin modulates miRNA expression, thereby inhibiting tumor formation. Together, our findings indicate that cardamonin has the potential to be considered for future therapy against colorectal cancer.

Keywords: azoxymethane; cardamonin; colitis; colorectal cancer; microRNA; nutraceutical.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Inflammatory Agents / administration & dosage*
  • Anti-Inflammatory Agents / pharmacology
  • Azoxymethane / adverse effects
  • Cell Proliferation / drug effects
  • Cell Survival / drug effects
  • Chalcones / administration & dosage*
  • Chalcones / pharmacology
  • Colitis / chemically induced
  • Colitis / complications
  • Colitis / drug therapy*
  • Colitis / metabolism
  • Colorectal Neoplasms / drug therapy*
  • Colorectal Neoplasms / etiology
  • Colorectal Neoplasms / genetics
  • Dextran Sulfate / adverse effects
  • Disease Models, Animal
  • Gene Expression Regulation, Neoplastic / drug effects
  • HCT116 Cells
  • Humans
  • Mice
  • MicroRNAs / genetics*
  • Nitrous Oxide / metabolism
  • RAW 264.7 Cells
  • Sequence Analysis, RNA
  • Signal Transduction / drug effects
  • THP-1 Cells


  • Anti-Inflammatory Agents
  • Chalcones
  • MicroRNAs
  • Dextran Sulfate
  • cardamonin
  • Nitrous Oxide
  • Azoxymethane