Soft-sensor development for monitoring the lysine fermentation process

J Biosci Bioeng. 2021 Aug;132(2):183-189. doi: 10.1016/j.jbiosc.2021.04.002. Epub 2021 May 3.


Monitoring cell growth and target production in working fermentors is important for stabilizing high level production. In this study, we developed a novel soft sensor for estimating the concentration of a target product (lysine), substrate (sucrose), and bacterial cell in commercially working fermentors using machine learning combined with available on-line process data. The lysine concentration was accurately estimated in both linear and nonlinear models; however, the nonlinear models were also suitable for estimating the concentrations of sucrose and bacterial cells. Data enhancement by time interpolation improved the model prediction accuracy and eliminated unnecessary fluctuations. Furthermore, the soft sensor developed based on the dataset of the same process parameters in multiple fermentor tanks successfully estimated the fermentation behavior of each tank. Machine learning-based soft sensors may represent a novel monitoring system for digital transformation in the field of biotechnological processes.

Keywords: Big data; Data science; Digital transformation; Fermentation; Lysine; Machine learning; Soft sensor.

MeSH terms

  • Bacteria
  • Bioreactors*
  • Biotechnology
  • Fermentation*
  • Lysine


  • Lysine