Antidiabetic activities of glycoprotein from pea (Pisum sativum L.) in STZ-induced diabetic mice

Food Funct. 2021 Jun 8;12(11):5087-5095. doi: 10.1039/d1fo00535a.

Abstract

Polysaccharides have hypoglycemic activity and pea protein has high nutritional value. The purified pea glycoprotein PGP2 has been shown to inhibit the activity of α-glucosidase and α-amylase in previous studies. To study the mechanism of PGP2-induced blood glucose lowering in vivo, this paper established a diabetic mouse model by intraperitoneal injection of STZ and high-fat diet, and evaluated the blood-glucose-lowering activity of the pea component PGP2 at different doses. The results showed that intragastric administration of PGP2 could effectively reduce diabetic weight loss and polyphagia symptoms, reduce fasting blood glucose levels in mice, and improve oral glucose tolerance levels in mice. PGP2 could promote insulin secretion and had a protective effect on mouse organs. After intragastric administration of PGP2 in mice, the serum levels of total cholesterol, triglycerides and low-density lipoprotein decreased. PGP2 up-regulated the gene expression of insulin receptor substrates IRS-1 and IRS-2 in liver tissues, thereby reducing insulin resistance. Based on the above experimental results, PGP2 had good hypoglycemic activity and was expected to be developed as a natural medicine for the treatment of type II diabetes.

MeSH terms

  • ATP Binding Cassette Transporter, Subfamily B / genetics
  • ATP Binding Cassette Transporter, Subfamily B / metabolism
  • ATP-Binding Cassette Sub-Family B Member 4
  • Animals
  • Blood Glucose / metabolism
  • Diabetes Mellitus, Experimental / drug therapy
  • Diabetes Mellitus, Type 2 / metabolism*
  • Diet, High-Fat / adverse effects
  • Glucose Tolerance Test
  • Glycoproteins / pharmacology*
  • Glycoproteins / therapeutic use
  • Hypoglycemic Agents / pharmacology*
  • Hypoglycemic Agents / therapeutic use
  • Insulin / blood
  • Insulin Receptor Substrate Proteins / metabolism
  • Liver / metabolism
  • Male
  • Mice
  • Mice, Inbred ICR
  • Pisum sativum / chemistry*
  • Triglycerides / blood

Substances

  • ATP Binding Cassette Transporter, Subfamily B
  • Blood Glucose
  • Glycoproteins
  • Hypoglycemic Agents
  • Insulin
  • Insulin Receptor Substrate Proteins
  • Triglycerides