Dual proteome-scale networks reveal cell-specific remodeling of the human interactome

Cell. 2021 May 27;184(11):3022-3040.e28. doi: 10.1016/j.cell.2021.04.011. Epub 2021 May 6.


Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 cells. These networks model the interactome whose structure encodes protein function, localization, and complex membership. Comparison across cell lines validates thousands of interactions and reveals extensive customization. Whereas shared interactions reside in core complexes and involve essential proteins, cell-specific interactions link these complexes, "rewiring" subnetworks within each cell's interactome. Interactions covary among proteins of shared function as the proteome remodels to produce each cell's phenotype. Viewable interactively online through BioPlexExplorer, these networks define principles of proteome organization and enable unknown protein characterization.

Keywords: AP-MS; BioPlex; bioinformatics; cell specificity; computational biology; human interactome; network biology; protein interactions; proteomics; proteotypes.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Computational Biology / methods
  • HCT116 Cells / metabolism
  • HEK293 Cells / metabolism
  • Humans
  • Mass Spectrometry / methods
  • Protein Interaction Mapping / methods*
  • Protein Interaction Maps / genetics*
  • Protein Interaction Maps / physiology
  • Proteome / genetics*
  • Proteome / metabolism
  • Proteomics / methods


  • Proteome