The effect of a plasma protein fraction on lipid synthesis in cultured skin fibroblasts from normals and patients with hyperapobetalipoproteinemia

Clin Invest Med. 1988 Apr;11(2):99-107.

Abstract

Lipid synthesis was measured in cultured skin fibroblasts obtained from normals and patients with Hyperapobetalipoproteinemia (HyperapoB). Using lipoprotein-deficient serum medium, triglyceride synthesis and cholesterol esterification were greater in normals than in HyperapoB due to differences in de novo synthesis, not to differences in re-esterification or to different rates of hydrolysis. When normal and HyperapoB cells were incubated in serum-free medium, however, lipid synthesis was the same. Serum was then fractionated chromatographically and a partially purified protein fraction shown to be responsible for the stimulatory effect in normals.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Apolipoproteins B / blood*
  • Blood Proteins / physiology*
  • Cells, Cultured
  • Fibroblasts / metabolism
  • Glucose / metabolism
  • Humans
  • Hyperlipoproteinemia Type II / blood*
  • Lipids / biosynthesis*
  • Male
  • Middle Aged
  • Oleic Acids / metabolism
  • Phospholipids / biosynthesis
  • Skin / metabolism
  • Triglycerides / biosynthesis

Substances

  • Apolipoproteins B
  • Blood Proteins
  • Lipids
  • Oleic Acids
  • Phospholipids
  • Triglycerides
  • Glucose