The purpose of our study was to determine the prevalence of urogenital mixed infections and the sensitivity of mycoplasmas and ureaplasmas to antibiotics in reproductive aged women with pelvic inflammatory disease. 4720 samples of biomaterial were obtained by urethral and cervical canal scrapings in 2360 women of reproductive age with pelvic inflammatory disease (2 samples from each woman). In 2360 samples, Chlamydia trachomatis, Trichomonas vaginalis and Gardnerella vaginalis were determined by multiplex PCR. 2360 samples were examined using the culture method.The cultivation, identification and susceptibility testing of urogenital mycoplasmas and ureaplasmas to 9 antibiotics were conducted with the use of commercial kits. In the study of 2360 samples of biomaterial by PCR, bacterial vaginosis (37.4%) was most often determined in women with PID.Chlamydiatrachomatiswas found in 8.3%, Trichomonasvaginalis- in 1.2% of women with PID. The cultivation and identification of urogenital mycoplasmas and ureaplasmas using biochemical markers revealed: Ureaplasma spp. in 543 women (23.0%) and Mycoplasma hominis in 179 women (0.7% of the total number of women examined). Number of women with mixed infection (positive results for Chlamydia trachomatis, Gardnerella vaginalis, Ureaplasma spp. andMycoplasma hominis) was 112. (4.7% of the total number of women with PID). The study of antibiotic sensitivity showed that most strains of Ureaplasma spp. and Mycoplasma hominis are highly susceptible to tetracycline antibiotics, especially doxycycline.