Nuclear expression of NHERF1/EBP50 in Clear Cell Renal Cell Carcinoma

Acta Histochem. 2021 Jul;123(5):151717. doi: 10.1016/j.acthis.2021.151717. Epub 2021 May 6.


The Na/H exchange regulatory factor 1 or Ezrin-radixin-moesin-binding phosphoprotein 50 (NHERF1/EBP50) is an adaptor protein implicated in the stabilization of molecular complexes linking extracellular signals with the cytoskeleton machinery. NHERF1 expression at the cell cortex is associated with the maintenance of adherent junction integrity in polarized epithelia. The role of NHERF1 in cancer depends on its localization within the cell, acting, in most cases, as a tumor suppressor when localized at the cell membrane, and as an oncogene, when expressed in the cytoplasm or the nucleus of cancer cells. The distribution of NHERF1 in renal cell carcinoma (RCC) has not been yet investigated. In this study, NHERF1 expression was examined by immunohistochemistry in papillary and clear cell RCC. We observed membranous staining in papillary RCC, whereas NHERF1 expression was nuclear and membranous in clear cell RCC. In comparison, NHERF1 immunohistochemistry in clear cell carcinomas of the ovary showed mainly nuclear staining. Our finding of the specific NHERF1 nuclear expression in clear cell carcinomas may help to elucidate the molecular changes that regulate its nuclear accumulation and to better understand its role in this cell compartment.

Keywords: Cancer; Clear cells; NHERF1; Nucleus; Ovarian; Renal.

MeSH terms

  • Adenocarcinoma / metabolism*
  • Biomarkers, Tumor / metabolism
  • Carcinoma, Renal Cell / metabolism*
  • Cell Membrane / metabolism
  • Cell Nucleus / metabolism*
  • Disease Progression
  • Female
  • Gene Expression Regulation, Neoplastic*
  • Humans
  • Immunohistochemistry
  • Ovarian Neoplasms / metabolism
  • Phosphoproteins / biosynthesis*
  • Phosphoproteins / metabolism
  • Prognosis
  • Retrospective Studies
  • Risk
  • Sodium-Hydrogen Exchangers / biosynthesis*


  • Biomarkers, Tumor
  • Phosphoproteins
  • Sodium-Hydrogen Exchangers
  • sodium-hydrogen exchanger regulatory factor