Polyherbal combination for wound healing: Matricaria chamomilla L. and Punica granatum L

Daru. 2021 Jun;29(1):133-145. doi: 10.1007/s40199-021-00392-x. Epub 2021 May 9.


Background: Punica granatum L. (pomegranate) with astringent activities and Matricaria chamomilla L. (chamomile) with anti-inflammatory and antioxidant properties are natural remedies used for various skin disorders, including wound healing.

Objectives: This study was conducted to evaluate the individual and combined wound healing activity of the methanol extracts of pomegranate and chamomile flowers.

Methods: After preparing the menthol fraction of pomegranate and chamomile flowers, the content of total phenols, total tannins, and total flavonoids of fractions was measured. For standardization of pomegranate and chamomile fractions, Gallic acid and apigenin-7-O-glucoside contents of them were determined using high-performance liquid chromatography (HPLC). Moreover, their antioxidant activities were examined using DPPH and FRAP tests. The antimicrobial assay was performed against Staphylococcus aureus, Staphylococcus epidermidis, and Pseudomonas aeruginosa. Three different concentrations of methanol fraction of each plant and one combination dose of fractions were investigated for their wound healing activities in an excision wound model on the rats' dorsum. Finally, histopathological studies were done at the end of the experiment.

Results: Phytochemical examinations showed high amounts of phenolic compounds in pomegranate flowers, while chamomile flower fractions contained a high amount of total flavonoids. Both fractions, especially pomegranate, had potent antioxidant activity. The best results for wound closure were observed 7 days after wound induction. All treated groups exhibited superior wound contraction compared to their placebo at all measurement times. The combined form of pomegranate and chamomile had better wound healing properties compared to a single therapy, especially on time earlier to wound induction.

Conclusion: This study represented high antioxidant and wound healing activities for methanol fraction of pomegranate and chamomile flowers, which could be related to their high content of phytochemicals. In comparison with single herb treatment, the combined form of these two fractions in lower concentrations accelerated wound closure.

Keywords: Antibacterial activity; Antioxidant activity; Matricaria chamomilla L.; Persian medicine; Punica granatum L.; Wound healing.

MeSH terms

  • Animals
  • Anti-Bacterial Agents / chemistry
  • Anti-Bacterial Agents / pharmacology
  • Anti-Bacterial Agents / therapeutic use*
  • Anti-Inflammatory Agents / pharmacology
  • Anti-Inflammatory Agents / therapeutic use*
  • Antioxidants / chemistry
  • Antioxidants / pharmacology
  • Antioxidants / therapeutic use*
  • Biphenyl Compounds / chemistry
  • Flavonoids / analysis
  • Flowers
  • Male
  • Matricaria*
  • Phenols / analysis
  • Phytochemicals / analysis
  • Phytochemicals / pharmacology
  • Phytochemicals / therapeutic use
  • Picrates / chemistry
  • Plant Extracts / chemistry
  • Plant Extracts / pharmacology
  • Plant Extracts / therapeutic use*
  • Pomegranate*
  • Pseudomonas aeruginosa / drug effects
  • Rats
  • Rats, Wistar
  • Skin / drug effects
  • Skin / pathology
  • Staphylococcus aureus / drug effects
  • Streptococcus / drug effects
  • Tannins / analysis
  • Wound Healing / drug effects*


  • Anti-Bacterial Agents
  • Anti-Inflammatory Agents
  • Antioxidants
  • Biphenyl Compounds
  • Flavonoids
  • Phenols
  • Phytochemicals
  • Picrates
  • Plant Extracts
  • Tannins
  • 1,1-diphenyl-2-picrylhydrazyl