Penetration of Singlet Oxygen into Films with Oxygen Permeability Coefficient Close to that of Skin

Abstract

Although its antiviral and antibacterial functions help prevent infection, singlet oxygen (¹ O₂ )-which is generated by the action of light on an endogenous photosensitizer-is cytotoxic. In the present study, we investigated the ability of ¹ O₂ -generated by the action of visible light on a photosensitizer-to penetrate skin. We used two polymer films with oxygen permeability coefficients similar to that of skin-i.e. cellulose acetate (CA) and ethyl cellulose (EC). Both films contained 1,3-diphenylisobenzofuran (DPBF), which was used as an ¹ O₂ probe. ¹ O₂ generated externally did not permeate the films by mere contact. Therefore, we conclude that the potential for ¹ O₂ to penetrate the skin is very low, and films that generate ¹ O₂ are safe and useful for preventing infections by contact. We also proved that ¹ O₂ can move between the layers of integrated polymer films when they are joined together.