Genome-wide identification and expression pattern analysis of lipoxygenase gene family in banana

Fan Liu; Hua Li; Junwei Wu; Bin Wang; Na Tian; Jiapeng Liu; Xueli Sun; Huan Wu; Yuji Huang; Peitao Lü; Chunzhen Cheng

doi:10.1038/s41598-021-89211-6

Genome-wide identification and expression pattern analysis of lipoxygenase gene family in banana

Sci Rep. 2021 May 11;11(1):9948. doi: 10.1038/s41598-021-89211-6.

Authors

Fan Liu^#^{1

2}, Hua Li^#¹, Junwei Wu^{1

2}, Bin Wang^{1

2}, Na Tian^{1

2}, Jiapeng Liu^{1

2}, Xueli Sun³, Huan Wu^{1

2}, Yuji Huang⁴, Peitao Lü⁵, Chunzhen Cheng^{6

7}

Affiliations

¹ College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
² College of Horticulture, Shanxi Agricultural University, Taigu, 030801, China.
³ College of Life Sciences, South China Agricultural University, Guangzhou, 510000, China.
⁴ College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, 350002, China. yjhuang2004@163.com.
⁵ College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, 350002, China. ptlv@fafu.edu.cn.
⁶ College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, 350002, China. ld0532cheng@126.com.
⁷ College of Horticulture, Shanxi Agricultural University, Taigu, 030801, China. ld0532cheng@126.com.

^# Contributed equally.

Abstract

The LOX genes have been identified and characterized in many plant species, but studies on the banana LOX genes are very limited. In this study, we respectively identified 18 MaLOX, 11 MbLOX, and 12 MiLOX genes from the Musa acuminata, M. balbisiana and M. itinerans genome data, investigated their gene structures and characterized the physicochemical properties of their encoded proteins. Banana LOXs showed a preference for using and ending with G/C and their encoded proteins can be classified into 9-LOX, Type I 13-LOX and Type II 13-LOX subfamilies. The expansion of the MaLOXs might result from the combined actions of genome-wide, tandem, and segmental duplications. However, tandem and segmental duplications contribute to the expansion of MbLOXs. Transcriptome data based gene expression analysis showed that MaLOX1, 4, and 7 were highly expressed in fruit and their expression levels were significantly regulated by ethylene. And 11, 12 and 7 MaLOXs were found to be low temperature-, high temperature-, and Fusarium oxysporum f. sp. Cubense tropical race 4 (FocTR4)-responsive, respectively. MaLOX8, 9 and 13 are responsive to all the three stresses, MaLOX4 and MaLOX12 are high temperature- and FocTR4-responsive; MaLOX6 and MaLOX17 are significantly induced by low temperature and FocTR4; and the expression of MaLOX7 and MaLOX16 are only affected by high temperature. Quantitative real-time PCR (qRT-PCR) analysis revealed that the expression levels of several MaLOXs are regulated by MeJA and FocTR4, indicating that they can increase the resistance of banana by regulating the JA pathway. Additionally, the weighted gene co-expression network analysis (WGCNA) of MaLOXs revealed 3 models respectively for 5 (MaLOX7-11), 3 (MaLOX6, 13, and 17), and 1 (MaLOX12) MaLOX genes. Our findings can provide valuable information for the characterization, evolution, diversity and functionality of MaLOX, MbLOX and MiLOX genes and are helpful for understanding the roles of LOXs in banana growth and development and adaptations to different stresses.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Fruit / metabolism
Gene Expression / genetics
Gene Expression Profiling / methods
Gene Expression Regulation, Plant / genetics
Genome-Wide Association Study / methods
Lipoxygenase / genetics*
Lipoxygenase / metabolism
Lipoxygenases / genetics
Lipoxygenases / metabolism
Musa / genetics*
Plant Diseases / genetics
Plant Roots / metabolism
Transcriptome / genetics

Substances

Lipoxygenases
Lipoxygenase