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. 2021 Jul 29;385(5):472-474.
doi: 10.1056/NEJMc2106083. Epub 2021 May 12.

BNT162b2-Elicited Neutralization against New SARS-CoV-2 Spike Variants

Yang Liu  1 , Jianying Liu  1 , Hongjie Xia  1 , Xianwen Zhang  1 , Jing Zou  1 , Camila R Fontes-Garfias  1 , Scott C Weaver  1 , Kena A Swanson  2 , Hui Cai  2 , Ritu Sarkar  2 , Wei Chen  2 , Mark Cutler  2 , David Cooper  2 , Alexander Muik  3 , Ugur Sahin  3 , Kathrin U Jansen  2 , Xuping Xie  4 , Philip R Dormitzer  5 , Pei-Yong Shi  6
Affiliations

BNT162b2-Elicited Neutralization against New SARS-CoV-2 Spike Variants

Yang Liu et al. N Engl J Med. .
No abstract available

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Figures

Figure 1
Figure 1. Serum Neutralization of New Variant Strains of SARS-CoV-2 after Two Doses of BNT162b2 Vaccine.
Shown are the results of 50% plaque reduction neutralization testing (PRNT50) with the use of 20 samples obtained from 15 trial participants at 2 weeks (circles) or 4 weeks (triangles) after the administration of the second dose of the BNT162b2 vaccine. The mutant viruses were produced by engineering the complete S genes from the B.1.429 variant (B.1.429-spike), B.1.526 variant (B.1.526-spike), or B.1.1.7 variant plus an additional E484K mutation (B.1.1.7-spike+E484K) into USA-WA1/2020. Each data point represents the geometric mean PRNT50 obtained with a serum sample against the indicated virus, including data from repeat experiments, as detailed in Table S1 in the Supplementary Appendix. The data for USA-WA1/2020 are from two experiments; the data for B.1.429-spike, B.1.526-spike, and B.1.1.7-spike+E484K viruses are from one experiment each. In each experiment, the neutralization titer was determined in duplicate assays, and the geometric mean was calculated. The heights of bars and the numbers over the bars indicate geometric mean titers. The 𝙸 bars indicate 95% confidence intervals. The dashed line indicates the limit of detection. Statistical analysis was performed with the use of the Wilcoxon matched-pairs signed-rank test. The statistical significance of the difference between geometric mean titers in the USA-WA1/2020 neutralization assay and in each variant virus neutralization assay with the same serum samples are as follows: P=0.002 for B.1.429-spike; P=0.47 for B.1.526-spike; and P=0.04 for B.1.1.7-spike+E484K.
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