Generation of a squamous cell carcinoma mouse model for lineage tracing of BMI1+ cancer stem cells

STAR Protoc. 2021 Apr 18;2(2):100484. doi: 10.1016/j.xpro.2021.100484. eCollection 2021 Jun 18.

Abstract

BMI1-expressing cancer stem cells (CSCs) play a key role in the development, progression, therapy resistance, recurrence, and metastasis of head and neck squamous cell carcinoma (HNSCC). Here, we present a chemically-induced HNSCC mouse model, genetically and pathologically similar to human HNSCC. This protocol describes how to use genetic lineage tracing based on the Cre-loxP recombination strategy, which allows us to study the regulation and targeting of BMI1+ CSCs in primary tumors and lymph node metastases. For complete details on the use and execution of this protocol, please refer to Chen et al. (2017) and Jia et al. (2020).

Keywords: Cancer; Microscopy; Model Organisms; Stem Cells.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Cell Lineage / genetics
  • Cells, Cultured
  • Female
  • Head and Neck Neoplasms* / genetics
  • Head and Neck Neoplasms* / metabolism
  • Head and Neck Neoplasms* / pathology
  • Immunohistochemistry
  • Male
  • Mice
  • Neoplasms, Experimental*
  • Neoplastic Stem Cells* / classification
  • Neoplastic Stem Cells* / cytology
  • Neoplastic Stem Cells* / metabolism
  • Polycomb Repressive Complex 1* / chemistry
  • Polycomb Repressive Complex 1* / metabolism
  • Proto-Oncogene Proteins* / chemistry
  • Proto-Oncogene Proteins* / metabolism
  • Squamous Cell Carcinoma of Head and Neck* / genetics
  • Squamous Cell Carcinoma of Head and Neck* / metabolism
  • Squamous Cell Carcinoma of Head and Neck* / pathology

Substances

  • Bmi1 protein, mouse
  • Proto-Oncogene Proteins
  • Polycomb Repressive Complex 1