A family of human CCAAT-box-binding proteins active in transcription and DNA replication: cloning and expression of multiple cDNAs

Nature. 1988 Jul 21;334(6179):218-24. doi: 10.1038/334218a0.


The CTF/NF-I group of cellular DNA binding proteins recognizes the sequence GCCAAT and is implicated in eukaryotic transcription as well as DNA replication. Molecular analysis of human CTF/NF-I complementary DNA clones reveals multiple messenger RNA species containing alternative coding regions, apparently as a result of differential splicing. Expression and functional analysis establish that individual gene products can bind to GCCAAT recognition sites and serve both as promoter-selective transcriptional activators and as initiation factors for DNA replication.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenoviridae / genetics
  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • DNA / genetics
  • DNA Replication*
  • DNA-Binding Proteins / genetics*
  • Humans
  • Molecular Sequence Data
  • Promoter Regions, Genetic
  • RNA Polymerase II / metabolism
  • RNA Splicing
  • Transcription Factors / genetics*
  • Transcription, Genetic*
  • Virus Replication


  • DNA-Binding Proteins
  • Transcription Factors
  • DNA
  • RNA Polymerase II

Associated data

  • GENBANK/X12492