Extracellular ATP-Induced Alterations in Extracellular H+ Fluxes From Cultured Cortical and Hippocampal Astrocytes

Abstract

Small alterations in the level of extracellular H⁺ can profoundly alter neuronal activity throughout the nervous system. In this study, self-referencing H⁺-selective microelectrodes were used to examine extracellular H⁺ fluxes from individual astrocytes. Activation of astrocytes cultured from mouse hippocampus and rat cortex with extracellular ATP produced a pronounced increase in extracellular H⁺ flux. The ATP-elicited increase in H⁺ flux appeared to be independent of bicarbonate transport, as ATP increased H⁺ flux regardless of whether the primary extracellular pH buffer was 26 mM bicarbonate or 1 mM HEPES, and persisted when atmospheric levels of CO₂ were replaced by oxygen. Adenosine failed to elicit any change in extracellular H⁺ fluxes, and ATP-mediated increases in H⁺ flux were inhibited by the P2 inhibitors suramin and PPADS suggesting direct activation of ATP receptors. Extracellular ATP also induced an intracellular rise in calcium in cultured astrocytes, and ATP-induced rises in both calcium and H⁺ efflux were significantly attenuated when calcium re-loading into the endoplasmic reticulum was inhibited by thapsigargin. Replacement of extracellular sodium with choline did not significantly reduce the size of the ATP-induced increases in H⁺ flux, and the increases in H⁺ flux were not significantly affected by addition of EIPA, suggesting little involvement of Na⁺/H⁺ exchangers in ATP-elicited increases in H⁺ flux. Given the high sensitivity of voltage-sensitive calcium channels on neurons to small changes in levels of free H⁺, we hypothesize that the ATP-mediated extrusion of H⁺ from astrocytes may play a key role in regulating signaling at synapses within the nervous system.

Keywords: ATP; H+; astrocyte; calcium; glia; pH.