Monoclonal antibody B72.3 reacts with a core region structure of O-linked carbohydrates

Tumour Biol. 1988;9(2-3):137-44. doi: 10.1159/000217554.


Monoclonal antibody (moAb) B72.3 appears to be exceptional among the many mouse moAbs reactive with human tumor cells in its degree of tumor specificity. The antigen recognized was characterized as a mucin-like molecule. We report here that B72.3 reacts strongly with ovine submaxillary mucin and that reactivity is abolished by desialylation, suggesting that the determinant recognized is the disaccharide N-acetylneuraminic acid alpha (2----6)-N-acetylgalactosamine, linked to serine or threonine, which might be designated sialylated Tn. A variety of human mucin preparations, including human salivary mucins, and other glycoproteins containing O-linked or N-linked carbohydrates were nonreactive. The data suggest that the determinant recognized is a core structure which is normally not exposed due to chain elongation at C-3 of the N-acetylgalactosamine, which appears to be less frequent in carcinoma cells than in normal cells.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology*
  • Antigens, Neoplasm / analysis*
  • Antigens, Neoplasm / immunology
  • Antigens, Tumor-Associated, Carbohydrate*
  • Carbohydrates / immunology*
  • Cattle
  • Glycoproteins / analysis
  • Humans
  • Mice
  • Mice, Inbred BALB C
  • Mucins / analysis
  • Mucins / immunology*
  • Pronase / pharmacology
  • Sheep


  • Antibodies, Monoclonal
  • Antigens, Neoplasm
  • Antigens, Tumor-Associated, Carbohydrate
  • Carbohydrates
  • Glycoproteins
  • Mucins
  • Tn antigen
  • Pronase