Objective: To observe the effect of Zhulian needle insertion by slow twirling technique on the contents of hydrogen peroxide (H2O2) and malondialdehyde (MDA) and apoptosis rate in kidney tissue of aging model rats and explore the potential mechanism of such needling technique.
Methods: A total of 40 adult male SD rats were randomized into a blank control group, a model control group, a Zhulian needling technique group and a routine acupuncture group, 10 rats in each one. Except the blank control group, the sub-acute aging rat models were established by intraperitoneal injection with 10% D-galactose solution, 5 mL/kg in the rats of the other groups. In the Zhulian needling technique group and the routine acupuncture group, Zhulian needle insertion by slow twirling technique and the routine acupuncture technique were exerted at "Guanyuan" (CV 4) and "Zusanli" (ST 36) respectively. The needles were retained for 30 min, once a day, for 28 days totally. After intervention, the content of H2O2 in kidney tissue was detected by colorimetry, the content of MDA in kidney tissue was detected by thiobarbituric acid method and the apoptosis rate of kidney cells was detected by terminal dexynucleotidyl transferase (TdT)-mediated dUTP nick end labeling.
Results: Compared with the blank control group, the contents of H2O2 and MDA in kidney tissue and kidney cell apoptosis rate were all increased in the model control group (P<0.05). Compared with the model control group, the contents of H2O2 and MDA in kidney tissue and kidney cell apoptosis rate were all reduced in the Zhulian needling technique group and the routine acupuncture group (P<0.05), and these indexes in the Zhulian needling technique group were all lower than the routine acupuncture group (P<0.05).
Conclusion: Zhulian needle insertion by slow twirling technique delays aging probably by regulating peroxidation and apoptosis.
目的：观察朱琏缓慢捻进针法对衰老模型大鼠肾组织过氧化氢（H2O2）、丙二醛（MDA）含量及细胞凋亡率的影响，探讨朱琏缓慢捻进针法延缓衰老的可能机制。方法：将40只成年雄性SD大鼠随机分成空白对照组、模型对照组、朱琏针法组和常规针刺组，每组10只。除空白对照组外，其余各组大鼠均按5 mL/kg的剂量腹腔注射浓度为10%的D-半乳糖溶液建立亚急性衰老大鼠模型。朱琏针法组和常规针刺组于“关元”和“足三里”穴分别行朱琏缓慢捻进针法、常规针刺法针刺，留针30 min，每日1次，共28 d。干预结束后，用比色法检测肾组织H2O2的含量，用硫代巴比妥酸法检测肾组织MDA的含量，用原位末端标记法检测肾细胞凋亡率。结果：与空白对照组比较，模型对照组肾组织H2O2和MDA的含量及肾细胞凋亡率升高(P<0.05)；与模型对照组比较，朱琏针法组和常规针刺组肾组织H2O2和MDA的含量以及肾细胞凋亡率均降低(P<0.05)，且朱琏针法组均低于常规针刺组(P<0.05)。结论：朱琏缓慢捻进针法可能通过调节肾组织过氧化反应和细胞凋亡延缓衰老。.
Keywords: Zhulian needle insertion by slow twirling technique; aging; apoptosis; hydrogen peroxide; malondialdehyde.