Serum biomarker discovery related to pathogenesis in acute coronary syndrome by proteomic approach

Biosci Rep. 2021 Jun 25;41(6):BSR20210344. doi: 10.1042/BSR20210344.

Abstract

Acute coronary syndrome (ACS) results from inadequate supply of blood flow from the coronary arteries to the heart or ischemia. ACS has an extremely high morbidity and mortality. The levels of biomarkers currently used for detection of ACS also increase in response to myocardial necrosis and other diseases and are not elevated immediately after symptoms appear, thus limiting their diagnostic capacity. Therefore, we aimed to discover new ACS diagnostic biomarkers with high sensitivity and specificity that are specifically related to ACS pathogenesis. Sera from 50 patients with ACS and healthy controls (discovery cohort) each were analyzed using mass spectrometry (MS) to identify differentially expressed proteins, and protein candidates were evaluated as ACS biomarkers in 120 people in each group (validation cohort). α-1-acid glycoprotein 1 (AGP1), complement C5 (C5), leucine-rich α-2-glycoprotein (LRG), and vitronectin (VN) were identified as biomarkers whose levels increase and gelsolin (GSN) as a biomarker whose levels decrease in patients with ACS. We concluded that these biomarkers are associated with the pathogenesis of ACS and can predict the onset of ACS prior to the appearance of necrotic biomarkers.

Keywords: acute coronary syndrome; biomarkers; pathogenesis; proteomics.

Publication types

  • Research Support, Non-U.S. Gov't
  • Validation Study

MeSH terms

  • Acute Coronary Syndrome / blood*
  • Acute Coronary Syndrome / diagnosis
  • Aged
  • Biomarkers / blood
  • Blood Proteins / analysis*
  • Case-Control Studies
  • Complement C5 / analysis
  • Female
  • Gelsolin / blood
  • Glycoproteins / blood
  • Humans
  • Male
  • Middle Aged
  • Orosomucoid / analysis
  • Predictive Value of Tests
  • Proteome*
  • Proteomics*
  • Reproducibility of Results
  • Tandem Mass Spectrometry
  • Vitronectin / blood

Substances

  • Biomarkers
  • Blood Proteins
  • Complement C5
  • Gelsolin
  • Glycoproteins
  • LRG1 protein, human
  • ORM1 protein, human
  • Orosomucoid
  • Proteome
  • Vitronectin