Outbreak of equine coronavirus infection among riding horses in Tokyo, Japan

Comp Immunol Microbiol Infect Dis. 2021 Aug;77:101668. doi: 10.1016/j.cimid.2021.101668. Epub 2021 May 10.

Abstract

In 2020, an outbreak of equine coronavirus (ECoV) infection occurred among 41 horses at a riding stable in Tokyo, Japan. This stable had 16 Thoroughbreds and 25 horses of other breeds, including Andalusians, ponies and miniature horses. Fifteen horses (37 %) showed mild clinical signs such as fever, lethargy, anorexia and diarrhoea, and they recovered within 3 days of onset. A virus neutralization test showed that all 41 horses were infected with ECoV, signifying that 26 horses (63 %) were subclinical. The results suggest that subclinical horses played an important role as spreaders. A genome sequence analysis revealed that the lengths from genes p4.7 to p12.7 or NS2 in ECoV differed from those of ECoVs detected previously, suggesting that this outbreak was caused by a virus different from those that caused previous outbreaks among draughthorses in Japan. Among 30 horses that tested positive by real-time RT-PCR, ECoV shedding periods of non-Thoroughbreds were significantly longer than those of Thoroughbreds. The difference in shedding periods may indicate that some breeds excrete ECoV longer than other breeds and can contribute to the spread of ECoV.

Keywords: Equine coronavirus; Japan; Outbreak; Subclinical infection; Thoroughbred.

MeSH terms

  • Animals
  • Betacoronavirus 1*
  • Coronavirus Infections* / epidemiology
  • Coronavirus Infections* / veterinary
  • Disease Outbreaks / veterinary
  • Horse Diseases* / epidemiology
  • Horses
  • Japan / epidemiology
  • Tokyo