Mechanisms of Enamel Mineralization Guided by Amelogenin Nanoribbons

J Dent Res. 2021 Dec;100(13):1434-1443. doi: 10.1177/00220345211012925. Epub 2021 May 19.


The nanofibrous nature and its intricate structural organization are the basis for the extraordinary ability of sound enamel to outlive masticatory forces at minimal failure rates. Apatite nanofibers of several hundreds of micrometers to possibly millimeters in length originate during the secretory stage of amelogenesis as 2-nm-thin and 15-nm-wide ribbons that develop and grow in length under the guidance of a dynamic mixture of specialized proteins, the developing enamel matrix (DEM). A critical role in the unidirectional and oriented growth of enamel mineral ribbons has been attributed to amelogenin, the major constituent of the DEM. This review elaborates on recent studies on the ability of ribbon-like assemblies of amelogenin to template the formation of an amorphous calcium phosphate precursor that transforms into apatite mineral ribbons similar to the ones observed in developing enamel. A mechanistic model of the biological processes that drive biomineralization in enamel is presented in the context of a comparative analysis of enamel mouse models and earlier structural data of the DEM emphasizing a regulatory role of the matrix metalloproteinase 20 in mineral deposition and the involvement of a process-directing agent for the templated mineral growth directed by amelogenin nanoribbons.

Keywords: amorphous calcium phosphate; apatite; biomineralization; developing enamel matrix; matrix metalloproteinase 20; self-assembly.

Publication types

  • Research Support, N.I.H., Extramural
  • Review

MeSH terms

  • Amelogenesis
  • Amelogenin
  • Animals
  • Dental Enamel
  • Dental Enamel Proteins*
  • Matrix Metalloproteinase 20
  • Mice
  • Nanotubes, Carbon*


  • Amelogenin
  • Dental Enamel Proteins
  • Nanotubes, Carbon
  • Matrix Metalloproteinase 20