ChIP-Sequencing of TET Proteins

Methods Mol Biol. 2021:2272:251-262. doi: 10.1007/978-1-0716-1294-1_15.

Abstract

TET proteins are methylcytosine dioxygenases that interact directly with chromatin to shape the DNA methylation landscape. To increase the understanding of TET protein function in a specific cellular context, it is important to be able to map the interactions between TET proteins and DNA. This ChIP-seq protocol details our procedure to analyze TET2 bound DNA in disuccinimidyl glutarate (DSG) and formaldehyde-crosslinked chromatin but can also be adapted to study other TET enzymes.

Keywords: ChIP-seq; DNA methylation; DSG; TET1; TET2; TET3.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatin Immunoprecipitation Sequencing / methods*
  • DNA Methylation*
  • Humans
  • Mixed Function Oxygenases / classification
  • Mixed Function Oxygenases / genetics
  • Mixed Function Oxygenases / metabolism*
  • Proto-Oncogene Proteins / classification
  • Proto-Oncogene Proteins / genetics
  • Proto-Oncogene Proteins / metabolism*
  • Sequence Analysis, DNA / methods*

Substances

  • Proto-Oncogene Proteins
  • Mixed Function Oxygenases
  • TET1 protein, human