Formation of acetaldehyde adducts with ethanol-inducible P450IIE1 in vivo

Biochem Biophys Res Commun. 1988 Jul 29;154(2):584-90. doi: 10.1016/0006-291x(88)90180-5.

Abstract

Hepatic microsomes, obtained from rats pair-fed liquid diets supplemented with either ethanol or an isocaloric amount of carbohydrates (for 4 weeks), were subjected to crossed immunoelectrophoresis. Anti-acetaldehyde adduct-specific immunoglobulin reacted on the protein blots with a single major 52,000 dalton polypeptide. This same protein was recognized by antibodies specific for P450IIE1, an ethanol-inducible P450 isozyme. Furthermore, a single protein, also reactive with anti-P450IIE1 IgG, was isolated from liver microsomes of ethanol-fed rats by immunoaffinity chromatography on Sepharose-conjugated anti-acetaldehyde adduct IgG. These results indicate that P450IIE1 is a target protein for acetaldehyde binding in liver microsomes in vivo.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Acetaldehyde / metabolism*
  • Animals
  • Cytochrome P-450 Enzyme System / biosynthesis*
  • Ethanol / pharmacology*
  • Isoenzymes / biosynthesis*
  • Male
  • Microsomes, Liver / drug effects
  • Microsomes, Liver / enzymology
  • Molecular Weight
  • Rats
  • Rats, Inbred Strains

Substances

  • Isoenzymes
  • Ethanol
  • Cytochrome P-450 Enzyme System
  • Acetaldehyde