Production of Mycophenolic Acid by a Newly Isolated Indigenous Penicillium glabrum

Curr Microbiol. 2021 Jun;78(6):2420-2428. doi: 10.1007/s00284-021-02509-6. Epub 2021 May 21.

Abstract

Soil-occupant fungi produce a variety of mycotoxins as secondary metabolites, one of which is mycophenolic acid (MPA), an antibiotic and immunosuppressive agent. MPA is mainly produced by several species of Penicillium, especially Penicillium brevicompactum. Here, we present the first report of MPA production by a local strain belonging to Penicillium glabrum species. We screened ascomycete cultures isolated from moldy food and fruits, as well as soils, collected from different parts of Iran. MPA production of one hundred and forty Penicillium isolates was analyzed using HPLC. Three MPA producer isolates were identified, among which the most producer was subjected to further characterization, based on morphological and microscopic analysis, as well as molecular approach (ITS, rDNA and beta-tubulin gene sequences). The results revealed that the best MPA producer belongs to P. glabrum IBRC-M 30518, and can produce 1079 mg/L MPA in Czapek-Dox medium.

MeSH terms

  • Iran
  • Mycophenolic Acid
  • Penicillium* / genetics

Substances

  • Mycophenolic Acid

Supplementary concepts

  • Penicillium brevicompactum
  • Penicillium glabrum