Egg white peptides ameliorate dextran sulfate sodium-induced acute colitis symptoms by inhibiting the production of pro-inflammatory cytokines and modulation of gut microbiota composition

Food Chem. 2021 Oct 30;360:129981. doi: 10.1016/j.foodchem.2021.129981. Epub 2021 May 12.


Egg white peptides (EWPs) can be effectively used to alleviate and treat inflammatory diseases due to their anti-oxidation, anti-inflammation, and microbiota regulation capabilities. A dextran sodium sulfate (DSS)-induced colitis model was used to clarify the regulatory effects of EWPs on colitis. Forty-three peptide sequences were identified from EWPs using LC-MS/MS. The results demonstrated that EWPs decreased the levels of pro-inflammatory cytokines and the extent of crypt damage in a dose-dependent manner. 16S rRNA gene sequencing results indicated that 200 mg/kg EWPs significantly increased the relative abundance of beneficial bacteria Lactobacillus and Candidatus_Saccharimonas, and reduced the relative abundance of pathogenic bacteria Ruminiclostridium and Akkermansia. In addition, the degree of correlation between pro-inflammatory cytokines and microbiota was as follows: interleukin (IL)-1β > IL-8 > IL-6 > tumor necrosis factor-α To summarize, EWPs contributed to the alleviation of colitis symptoms and the intestinal injury through anti-inflammatory effects, repair of intestinal mucosa, and modulation of gut microbiota.

Keywords: Acute colitis; Dextran sulfate sodium (DSS); Egg white peptides (EWPs); Gut microbiota; Pro-inflammatory cytokines.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Bacteria / classification
  • Bacteria / genetics
  • Bacteria / isolation & purification
  • Chromatography, High Pressure Liquid
  • Colitis / chemically induced
  • Colitis / drug therapy
  • Colitis / microbiology
  • Colitis / pathology
  • Colon / drug effects
  • Colon / metabolism*
  • Colon / microbiology
  • Colon / pathology
  • Cytokines / metabolism*
  • Dextran Sulfate / toxicity
  • Egg Proteins / chemistry
  • Egg Proteins / metabolism
  • Egg White / chemistry*
  • Gastrointestinal Microbiome / drug effects*
  • Lactobacillus / genetics
  • Lactobacillus / isolation & purification
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Peptides / analysis
  • Peptides / pharmacology*
  • Peptides / therapeutic use
  • RNA, Ribosomal, 16S / analysis
  • RNA, Ribosomal, 16S / metabolism
  • Tandem Mass Spectrometry


  • Cytokines
  • Egg Proteins
  • Peptides
  • RNA, Ribosomal, 16S
  • Dextran Sulfate