Sequencing and analysis of the complete mitochondrial genome of Micromys erythrotis from China and its phylogenetic analysis

He Cai; Qing-Qing Wang; Xin-Xu Zhao; Qian-Qian Yao; Ning Wu; Jun-Sheng Zhang; Zhu Liu

doi:10.1080/23802359.2021.1926353

Sequencing and analysis of the complete mitochondrial genome of Micromys erythrotis from China and its phylogenetic analysis

Mitochondrial DNA B Resour. 2021 May 12;6(5):1617-1620. doi: 10.1080/23802359.2021.1926353.

Authors

He Cai¹, Qing-Qing Wang¹, Xin-Xu Zhao¹, Qian-Qian Yao¹, Ning Wu², Jun-Sheng Zhang¹, Zhu Liu¹

Affiliations

¹ College of Life Science and Technology, Mudanjiang Normal University, Mudanjiang, P.R. China.
² Ningan Jiangnan School, Ningan, P.R. China.

Abstract

The complete mitogenome sequence of Micromys erythrotis was determined using long PCR. The genome was 16,238 bp in length and contained 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes, 1 origin of L strand replication and 1 control region. The overall base composition of the heavy strand is A (33.7%), C (24.8%), T (29.1%) and G (12.4%). The base compositions present clearly the A-T skew, which is most obviously in the control region and protein-coding genes. Mitochondrial genome analyses based on MP, ML, NJ and Bayesian analyses yielded identical phylogenetic trees. This study verifies the evolutionary status of Micromys erythrotis in Muridae at the molecular level. The mitochondrial genome would be a significant supplement for the Micromys erythrotis genetic background.

Keywords: Control region; Micromys erythrotis; mitogenome; phylogenetic trees.

Grants and funding

This research was supported by the Heilongjiang Provincial Department of Education filing project [1353MSYQN013; 1355ZD007; 1355JG002]; the Project of Mudanjiang Normal University [GP2020002].