Synthesis of Profluorescent Strigolactone Probes for Biochemical Studies

Alexandre de Saint Germain; Guillaume Clavé; François-Didier Boyer

doi:10.1007/978-1-0716-1429-7_17

Synthesis of Profluorescent Strigolactone Probes for Biochemical Studies

Methods Mol Biol. 2021:2309:219-231. doi: 10.1007/978-1-0716-1429-7_17.

Authors

Alexandre de Saint Germain¹, Guillaume Clavé², François-Didier Boyer³

Affiliations

¹ Institut Jean-Pierre Bourgin, INRAE, AgroParisTech, Université Paris-Saclay, Versailles, France.
² Université Paris-Saclay, CNRS, Institut de Chimie des Substances Naturelles, Gif-sur-Yvette, France.
³ Université Paris-Saclay, CNRS, Institut de Chimie des Substances Naturelles, Gif-sur-Yvette, France. francois-didier.boyer@cnrs.fr.

PMID: 34028690
DOI: 10.1007/978-1-0716-1429-7_17

Abstract

In this chapter, we will describe a method we set up to synthesize two profluorescent strigolactone (SL) mimic probes (GC240 and GC242) and the optimized protocols developed to study the enzymatic properties of various strigolactone receptors. The Arabidopsis AtD14 SL receptor is used here as a model for this purpose.

Keywords: Bioactive profluorescent probes; Enzymatic properties; Fluorescence; Receptor; Strigolactones; α/β Hydrolase.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Arabidopsis / drug effects
Arabidopsis / genetics
Arabidopsis / metabolism*
Arabidopsis Proteins / drug effects
Arabidopsis Proteins / genetics
Arabidopsis Proteins / metabolism*
Biological Assay*
Fluorescent Dyes / chemical synthesis*
Heterocyclic Compounds, 3-Ring / metabolism*
High-Throughput Screening Assays
Lactones / metabolism*
Luminescent Measurements
Plant Growth Regulators / pharmacology
Receptors, Cell Surface / drug effects
Receptors, Cell Surface / genetics
Receptors, Cell Surface / metabolism*
Signal Transduction

Substances

Arabidopsis Proteins
DWARF14 protein, Arabidopsis
Fluorescent Dyes
GR24 strigolactone
Heterocyclic Compounds, 3-Ring
Lactones
Plant Growth Regulators
Receptors, Cell Surface