Mouse Models of Kidney Fibrosis

Methods Mol Biol. 2021;2299:323-338. doi: 10.1007/978-1-0716-1382-5_22.

Abstract

Chronic kidney disease (CKD) affects over 10% of the worldwide population and kidney fibrosis is a main driver of CKD and considered a therapeutic target. The mechanisms leading to kidney fibrosis are highly complexed and can be best studied in rodent models. Here we describe the most commonly used kidney fibrosis models in mice, the unilateral ureteral obstruction (UUO) model and the ischemia reperfusion injury (IRI) model. Both models are easy to learn and can be applied in animals of different age, sex, and strain.

Keywords: Hydronephrosis; IRI; Mouse model; UUO; Unilateral ischemia reperfusion.

MeSH terms

  • Animals
  • Biomarkers / metabolism
  • Disease Models, Animal
  • Female
  • Fibrosis
  • Humans
  • Kidney / metabolism
  • Kidney / pathology*
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Reperfusion Injury / metabolism
  • Reperfusion Injury / pathology*
  • Ureteral Obstruction / metabolism
  • Ureteral Obstruction / pathology*

Substances

  • Biomarkers