Age and sex-specific reference intervals for pro-oxidant-antioxidant balance (PAB), anti-heat-shock protein 27 (Anti-hsp27) and routine laboratory tests in the middle-aged adult population

Biotechnol Appl Biochem. 2021 May 24. doi: 10.1002/bab.2203. Online ahead of print.


Introduction: We aimed to define specific reference intervals (RIs) for eleven biomarkers including inflammatory and oxidative stress biomarkers, liver and renal function tests in a healthy Iranian adult population for the first time.

Methods: CLSI Ep28-A3 guidelines were then used to calculate accurate age- and sex- as well as BMI-specific RIs.

Results: RIs for studied biomarkers showed no significant age and sex-specific differences, except for uric acid, which had higher concentrations in men when compared to women. Additionally, after partitioning the participants based on the body mass index (BMI) with a cutoff point of 25 kg/m2 , only the levels of hs-CRP were positively associated with higher BMI (RI for BMI>25: 0.51 - 7.85 mg/L and for BMI< 25: 0.40 - 4.46 mg/L). RI for PAB and anti hsp-27 were reported 4.69-155.36 HK and 0.01-0.70 OD in men and women aged 35 to 65 years old.

Conclusion: Partitioning by sex and BMI was only required for uric acid and hs-CRP, respectively, while other biomarkers required no partitioning. These results can be expected to valuably contribute to improve laboratory test result interpretation in adult for improved monitoring of various diseases in the Iranian population. This article is protected by copyright. All rights reserved.

Keywords: Anti-heat-shock protein 27; Pro-oxidant-antioxidant balance; high sensitivity C-reactive protein; laboratory biomarkers; reference interval.