Zebrafish Tumor Graft Transplantation to Grow Tumors In Vivo That Engraft Poorly as Single Cell Suspensions

Zebrafish. 2021 Aug;18(4):293-296. doi: 10.1089/zeb.2021.0006. Epub 2021 May 24.


Angiosarcoma is a clinically aggressive tumor with a high rate of mortality. It can arise in vascular or lymphatic tissues, involve any part of the body, and aggressively spread locally or metastasize. Angiosarcomas spontaneously develop in the tp53 deleted (tp53del/del) zebrafish mutant. However, established protocols for tumor dissection and transplantation of single cell suspensions of angiosarcoma tumors result in inferior implantation rates. To resolve these complications, we developed a new tumor grafting technique for engraftment of angiosarcoma and similar tumors in zebrafish, which maintains the tumor microenvironment and has superior rates of engraftment.

Keywords: angiosarcoma; tp53; tumor graft; zebrafish; zebrafish cancer model.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Disease Models, Animal
  • Hemangiosarcoma* / pathology
  • Neoplasm Transplantation*
  • Suspensions
  • Tumor Microenvironment
  • Zebrafish*


  • Suspensions